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BMG Labtech
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Bio-Rad
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GraphPad Software Inc
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GE Healthcare
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Image Search Results
Journal: PLoS ONE
Article Title: High Affinity Binders to EphA2 Isolated from Abdurin Scaffold Libraries; Characterization, Binding and Tumor Targeting
doi: 10.1371/journal.pone.0135278
Figure Lengend Snippet: hEph2A was immobilized on CM5 chip by amine coupling with a ligand density of 500 RU. Serial dilutions of analyte in HBS-P running buffer were injected over the ligand (2.5 min association time, 5 min dissociation time) followed by surface regeneration. Sensorgrams for: (A) G7 (3.7 to 300 nM), (B) B6 (9 to 150 nM), (C) B11 (1.56 to 100 nM), and (D) B11 (red sensorgram) or shWTCH2 (black sensorgram), were analyzed with BiaEvaluation software v 3.0 and kinetic parameters or affinity at the equilibrium were evaluated.
Article Snippet: The binding of Abdurins to the immobilized ligand was evaluated by a multi-cycle kinetic procedure in
Techniques: Injection, Software